Researchers used advanced computational tools (QENIE and GD-CAT) on multi-tissue data from the MoTrPAC rat endurance training study to map how 8 weeks of treadmill running reshapes hormonal communication between organs. Subcutaneous white adipose tissue emerged as the most influential endocrine hub after training, with its signaling to skeletal muscle showing the greatest change. Extracellular matrix proteins and Wnt signaling factors were identified as globally regulated secretory mediators. Well-known exercise signals like leptin, IL-15, and TGF-β2 were validated, while new tissue crosstalk candidates were uncovered. The resulting atlas provides a resource for discovering novel exerkines and understanding how chronic exercise produces systemic health benefits.
